Crosslinking and splitting proteins
In processing meat and meat products, manufacturers try to use the slaughtered animals as completely as possible. In doing so, enzymes are very important and efficient aids for enabling this. In addition to processing meat by-products like organs and bones, manufacturers aim at maximising the meat yield by using residual meat sticking to the bone, and also at adjusting certain characteristics of the final products.
Two methods are frequently used, crosslinking and splitting linkages in meat proteins, known as peptide bonds. Using transglutaminase, proteins can be specifically crosslinked in order to reconstitute residual meat pieces, making it accessible for human consumption.
On the other hand, by selectively breaking down peptide bonds in myofibrils and connective tissue, meat can be tenderised.
Transglutaminase crosslinks proteins to reconstitute meat pieces or to improve the texture of sausage products.
Plant proteases and microbial Collagenases break down myofibril and connective tissue proteins to tenderise meat.
The complete utilization of meat components is imperative for responsible economy. Reducing energy use in the processing of meat and meat by-products plays an important role in this. Special enzymes are very efficient tools for the sustainable use of raw materials from animal sources, and for reducing curing and cooking times.
Sven Konradt, Head of Research and Development
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Protein crosslinking
Transglutaminase creates new linkages within and between proteins. The resulting peptide bonds link primarily at the ends of the amino acids L-glutamine and L-lysine. The meat industry makes use of this function to combine individual meat pieces, improve the texture of sausage products, and improve the cohesion of sausage slices.
The use of microbial transglutaminases can thus maximise meat yield and optimise the characteristics of meat products.
Splitting proteins
The toughness of insufficiently matured meat is due primarily to myofibrillar proteins and structural proteins in connective tissue. The use of enzymes to break down these usually very resistant proteins makes the meat tender, juicier, and generally better tasting.
Plant proteases like papain and bromelain are familiar meat tenderisers and thus play a key role. Microbial Collagenases are also used to selectively break down collagen, the main protein in connective tissue.